| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
29/06/2017 |
|
Data da última atualização: |
29/06/2017 |
|
Autoria: |
KNIGHT, P. G.; BEARD, A. J.; WRATHALL, J. H.; CASTILLO, R. J. |
|
Título: |
Evidence that the bovine ovary secretes large amounts of monomeric inhibin alpha subunit and its isolation from bovine follicular fluid. |
|
Ano de publicação: |
1989 |
|
Fonte/Imprenta: |
Journal of Molecular Endocrinology, v. 2, n. 3, p. 189-200, May, 1989. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Analysis of bovine follicular fluid (FF) using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) coupled with a sensitive immunoblotting procedure resolved several components that were immunoreactive with an antiserum directed against the n-terminus of the alpha subunit of human inhibin (hI alpha(1-32]. Under non-reducing conditions, three intensely stained bands having apparent Mr values of 116,000, 44,000 and 25,000 were present, whilst under reducing conditions only two intensely stained bands (Mr 43,000 and 21,000) were detected. The Mr 44,000 and 25,000 immunoreactive forms (non-reducing conditions) were also demonstrated in bovine utero-ovarian vein and peripheral venous plasma after subjecting samples (40 ml) to immunoaffinity concentration using Sepharose beads coupled to anti-hI alpha(1-32), SDS-PAGE and immunoblotting. The same approach revealed the presence of the smaller (Mr 25,000) form in bovine granulosa cell-conditioned culture medium (GCCM). Gel-permeation chromatography (Sephacryl S-200), immunoaffinity chromatography (Sepharose-anti-hI alpha(1-32] and reversed-phase high-performance liquid chromatography (RP-HPLC; C18 and C8 columns) were employed to isolate from bFF (30 ml, 19.5 g protein) 750 micrograms protein which appeared essentially homogeneous by RP-HPLC and SDS-PAGE and had an Mr of 25,000 (non-reducing conditions)/21,000 (reducing conditions), identical to that of the immunoreactive component of lowest Mr found in bovine FF, utero-ovarian vein plasma, peripheral plasma and GCCM. The isolated material was highly immunoreactive with antisera against both hI alpha(1-32) and purified Mr 32,000 bovine inhibin but was devoid of biological activity when tested in a rat pituitary cell inhibin bioassay. Amino-terminal analysis revealed an amino acid sequence (residues 1-14) identical to that reported elsewhere for the alpha subunit (Mr 20,000/21,000) of bovine inhibin. In conclusion, the present study has revealed that the bovine ovary secretes considerable quantities of monomeric inhibin alpha subunit. The unexpected presence of this material in peripheral blood is likely to hinder attempts to obtain physiologically relevant data on circulating levels of inhibin in cattle using conventional radioimmunoassays. MenosAbstract: Analysis of bovine follicular fluid (FF) using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) coupled with a sensitive immunoblotting procedure resolved several components that were immunoreactive with an antiserum directed against the n-terminus of the alpha subunit of human inhibin (hI alpha(1-32]. Under non-reducing conditions, three intensely stained bands having apparent Mr values of 116,000, 44,000 and 25,000 were present, whilst under reducing conditions only two intensely stained bands (Mr 43,000 and 21,000) were detected. The Mr 44,000 and 25,000 immunoreactive forms (non-reducing conditions) were also demonstrated in bovine utero-ovarian vein and peripheral venous plasma after subjecting samples (40 ml) to immunoaffinity concentration using Sepharose beads coupled to anti-hI alpha(1-32), SDS-PAGE and immunoblotting. The same approach revealed the presence of the smaller (Mr 25,000) form in bovine granulosa cell-conditioned culture medium (GCCM). Gel-permeation chromatography (Sephacryl S-200), immunoaffinity chromatography (Sepharose-anti-hI alpha(1-32] and reversed-phase high-performance liquid chromatography (RP-HPLC; C18 and C8 columns) were employed to isolate from bFF (30 ml, 19.5 g protein) 750 micrograms protein which appeared essentially homogeneous by RP-HPLC and SDS-PAGE and had an Mr of 25,000 (non-reducing conditions)/21,000 (reducing conditions), identical to that of the immunoreactive component of lowest Mr found in bo... Mostrar Tudo |
|
Palavras-Chave: |
Inhibins; Ovaries. |
|
Thesaurus Nal: |
Blood; Cattle; Granulosa Cells; Immunoblotting; Ovarian follicles; Polyacrylamide gel electrophoresis; Secretion; Sheep; Swine. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03134naa a2200289 a 4500
001 2071710
005 2017-06-29
008 1989 bl uuuu u00u1 u #d
100 1 $aKNIGHT, P. G.
245 $aEvidence that the bovine ovary secretes large amounts of monomeric inhibin alpha subunit and its isolation from bovine follicular fluid.
260 $c1989
520 $aAbstract: Analysis of bovine follicular fluid (FF) using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) coupled with a sensitive immunoblotting procedure resolved several components that were immunoreactive with an antiserum directed against the n-terminus of the alpha subunit of human inhibin (hI alpha(1-32]. Under non-reducing conditions, three intensely stained bands having apparent Mr values of 116,000, 44,000 and 25,000 were present, whilst under reducing conditions only two intensely stained bands (Mr 43,000 and 21,000) were detected. The Mr 44,000 and 25,000 immunoreactive forms (non-reducing conditions) were also demonstrated in bovine utero-ovarian vein and peripheral venous plasma after subjecting samples (40 ml) to immunoaffinity concentration using Sepharose beads coupled to anti-hI alpha(1-32), SDS-PAGE and immunoblotting. The same approach revealed the presence of the smaller (Mr 25,000) form in bovine granulosa cell-conditioned culture medium (GCCM). Gel-permeation chromatography (Sephacryl S-200), immunoaffinity chromatography (Sepharose-anti-hI alpha(1-32] and reversed-phase high-performance liquid chromatography (RP-HPLC; C18 and C8 columns) were employed to isolate from bFF (30 ml, 19.5 g protein) 750 micrograms protein which appeared essentially homogeneous by RP-HPLC and SDS-PAGE and had an Mr of 25,000 (non-reducing conditions)/21,000 (reducing conditions), identical to that of the immunoreactive component of lowest Mr found in bovine FF, utero-ovarian vein plasma, peripheral plasma and GCCM. The isolated material was highly immunoreactive with antisera against both hI alpha(1-32) and purified Mr 32,000 bovine inhibin but was devoid of biological activity when tested in a rat pituitary cell inhibin bioassay. Amino-terminal analysis revealed an amino acid sequence (residues 1-14) identical to that reported elsewhere for the alpha subunit (Mr 20,000/21,000) of bovine inhibin. In conclusion, the present study has revealed that the bovine ovary secretes considerable quantities of monomeric inhibin alpha subunit. The unexpected presence of this material in peripheral blood is likely to hinder attempts to obtain physiologically relevant data on circulating levels of inhibin in cattle using conventional radioimmunoassays.
650 $aBlood
650 $aCattle
650 $aGranulosa Cells
650 $aImmunoblotting
650 $aOvarian follicles
650 $aPolyacrylamide gel electrophoresis
650 $aSecretion
650 $aSheep
650 $aSwine
653 $aInhibins
653 $aOvaries
700 1 $aBEARD, A. J.
700 1 $aWRATHALL, J. H.
700 1 $aCASTILLO, R. J.
773 $tJournal of Molecular Endocrinology$gv. 2, n. 3, p. 189-200, May, 1989.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Solos. |
|
Data corrente: |
27/02/2009 |
|
Data da última atualização: |
11/04/2022 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
SANTOS, F. A. dos; RONA, F. B.; SERRENHO, F. G.; ALCANTARA, S. M.; PEREZ, D. V. |
|
Afiliação: |
FERNANDA ARDILHA DOS SANTOS, CNPQ; GERMANA BREVES RONA, CNPQ; FERNANDA GONÇALVES SERRENHO, FAPERJ; SARAI MARIA DE ALCANTARA, UFRJ; DANIEL VIDAL PEREZ, CNPS. |
|
Título: |
Efeito residual da aplicação de lodo de esgoto nas propriedades químicas de um Latossolo Vermelho. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
In: JORNADA GIULIO MASSARANI DE INICIAÇÃO CIENTÍFICA, ARTÍSTICA E CULTURAL DA UFRJ, 30., 2008, Rio de Janeiro. Livro de resumos [...]. Rio de Janeiro: Universidade Federal do Rio de Janeiro, 2008. p. 250. Ref. 707. |
|
Idioma: |
Português |
|
Conteúdo: |
O objetivo deste trabalho é estudar o impacto causado pelo uso agrícola do lodo de esgoto, de origem doméstica/industrial, nas variações de pH, CTC, C.Org., P, N desde a última aplicação deste material, em 2003. |
|
Palavras-Chave: |
Lodo de esgoto; Química ambiental. |
|
Thesagro: |
Latossolo Vermelho. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/339986/1/Efeito-residual-da-aplicacao-de-lodo-de-esgoto-2008.pdf
|
|
Marc: |
LEADER 00997nam a2200193 a 4500
001 1339986
005 2022-04-11
008 2008 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. A. dos
245 $aEfeito residual da aplicação de lodo de esgoto nas propriedades químicas de um Latossolo Vermelho.$h[electronic resource]
260 $aIn: JORNADA GIULIO MASSARANI DE INICIAÇÃO CIENTÍFICA, ARTÍSTICA E CULTURAL DA UFRJ, 30., 2008, Rio de Janeiro. Livro de resumos [...]. Rio de Janeiro: Universidade Federal do Rio de Janeiro, 2008. p. 250. Ref. 707.$c2008
520 $aO objetivo deste trabalho é estudar o impacto causado pelo uso agrícola do lodo de esgoto, de origem doméstica/industrial, nas variações de pH, CTC, C.Org., P, N desde a última aplicação deste material, em 2003.
650 $aLatossolo Vermelho
653 $aLodo de esgoto
653 $aQuímica ambiental
700 1 $aRONA, F. B.
700 1 $aSERRENHO, F. G.
700 1 $aALCANTARA, S. M.
700 1 $aPEREZ, D. V.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Solos (CNPS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | 1250047, CEPALINDEX; RESUMENES DE DOCUMENTOS CEPAL/ILPES, Comision Economica para America Latina. Centro Latino Americano de Documentacion Economica y Social, Santiago-Chile
Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Acre; Embrapa Amazônia Oriental; Embrapa Meio-Norte; Embrapa Soja. |   |
| Registros recuperados : 1 | |
|
|
|
